Hematology Methods for the Office Laboratory

Table of Contents

Hematology Methods for the Office Laboratory

“Education is a technique employed to open minds so that they may go from profound ignorance to thoughtful uncertainty.” 

A complete blood count is indicated for:

So, what’s in a CBC?

Collection Methods for Hematological Specimens

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S-Monovette(R) with EDTA

Automatic Tube Rocker

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Fibrinogen Determination

 Fibrinogen mg/dl= TS mg/dl(non-incubated) -TS mg/dl (incubated)

Platelet Counting Methods

Electronic Impedance Analyzers

How does it work?

Impedance Instruments for Veterinary Use

Impedance Analyzers

Examples of Impedance Analyzers

Examples of Impedance Analyzers

Advantages of Impedance Instruments

Disadvantages

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Buffy Coat Analysis

Examples of Buffy Coat Analyzers

Advantages of Buffy Coat Analyzers

Disadvantages

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The Importance of Blood Smears 

The Importance of Blood Smears

Abnormalities Not Routinely Found in “Auto-Diffs”

Abnormalities Not Routinely Found in “Auto-diffs”

Abnormalities Not Routinely Found in “Auto-diffs”

Abnormalities Not Routinely Found in “Auto-diffs”

Three Step Stains

Wright’s or Wright-Giemsa Stain

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Reticulocyte Count

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% Retics(corrected) = %Retics (observed) x Patient’s PCV Normal PCV

Quality Control

Common Causes of Erroneous Lab Results

Clinical laboratory Improvement Act of 1988 (CLIA) 

Which one’s right for me?

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Which one’s right for me?

The Five “Truths” for In-House Hematology

“When the person with money meets a person with experience, the person with the experience winds up with the money and the person with the money winds up with the experience.” -Harvey MacKay

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